Human Colonic Fibroblasts cells人原代结肠成纤维细胞 BioVector NTCC质粒载体菌种细胞基因保藏中心

BioVector® NTCC® Human Colonic Fibroblasts cells人原代结肠成纤维细胞

Product Description

Human Primary Colonic Fibroblasts from NTCC® are isolated from normal human colon tissue. Human Primary Colonic Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in NTCC®’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 1 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x106 cells per ml.

Product Testing

Human Primary Colonic Fibroblasts from NTCC® are tested for negative expression of von Willebrand Factor Expression/Factor VIII, cytokeratin 18, and alpha smooth muscle actin. Human Primary Colonic Fibroblasts are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by NTCC®. Repeated freezing and thawing of cells is not recommended.

Laboratory Applications

Human Primary Colonic Fibroblasts can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.

Storage of NTCC®’ Products

NTCC® ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C.

Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net