pM-CBE植物基因编辑质粒BioVector® CRISPR载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

BioVector® NTCC® pM-CBE植物基因编辑质粒BioVector® CRISPR载体

35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding CBE in place of viral GP
Vector backbonepCB301-2μ-HDV
Vector typePlant Expression, CRISPR
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Growth Temperature37°C
Growth Strain(s)DH5alpha
Copy numberLow Copy
Gene/Insert
Gene/Insert nameFull length TSWV M antigenome encoding CBE in place of viral GP
SpeciesSynthetic
Promoterduplicated cauliflower mosaic virus 35S promoter
Tag / Fusion Protein
3×flag (N terminal on insert)
Cloning Information
Cloning methodLigation Independent Cloning
5′ sequencing primer35S promoter/forward primer: CTATCCTTCGCAAGACCCTTC
3′ sequencing primerNos terminator/reverse primer: TCATCGCAAGACCGGCAA

Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net