PB-Pemax转座子基因编辑质粒BioVector®载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

BioVector® NTCC® PB-Pemax转座子基因编辑质粒BioVector®载体

All-in-one prime editor piggyBac transposon with Pemax

pEX-A2
Backbone manufacturerEurofins Genomics
Backbone size w/o insert (bp)5120
Total vector size (bp)13175
Modifications to backboneaddition of 3' and 5' piggyBac repeats, addition of pU6-pegRNA(B12B) sequence, addition of CAG promoter, addition of bGH (pA)
Vector typeMammalian Expression, CRISPR, Synthetic Biology ; piggyBac transposon
Selectable markersPuromycin ; thymidine kinase
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Temperature30°C
Growth Strain(s)NEB Stable
Growth instructionsIt is highly recommened to use NEB Stable at 30°C. Cloning efficiency and stability of plasmid can be compromised when using other strains / higher temperatures!
Copy numberUnknown
Gene/Insert
Gene/Insert nameSpCas9_H840A_KK-MMLV-RT_co-P2A-PAC_dTK
SpeciesSynthetic
Insert Size (bp)8055
PromoterCAG
Tags / Fusion Proteins
BPSV40 NLS (N terminal on insert)
BPSV40 NLS (C terminal on insert)
c-Myc NLS (C terminal on insert)
Cloning Information
Cloning methodLigation Independent Cloning
5′ sequencing primerGGGCAAGTCCAAGAAACTG

Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net