PB-Pemax转座子基因编辑质粒BioVector®载体 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格:¥39850
- 货 号:PB-PEmax
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
电话:400-800-2947 工作QQ:1843439339 (微信同号)
邮件:Biovector@163.com
手机:18901268599
地址:北京
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BioVector® NTCC® PB-Pemax转座子基因编辑质粒BioVector®载体
All-in-one prime editor piggyBac transposon with PemaxpEX-A2Backbone manufacturerEurofins GenomicsBackbone size w/o insert (bp)5120Total vector size (bp)13175Modifications to backboneaddition of 3' and 5' piggyBac repeats, addition of pU6-pegRNA(B12B) sequence, addition of CAG promoter, addition of bGH (pA)Vector typeMammalian Expression, CRISPR, Synthetic Biology ; piggyBac transposonSelectable markersPuromycin ; thymidine kinaseGrowth in BacteriaBacterial Resistance(s)Ampicillin, 100 μg/mLGrowth Temperature30°CGrowth Strain(s)NEB StableGrowth instructionsIt is highly recommened to use NEB Stable at 30°C. Cloning efficiency and stability of plasmid can be compromised when using other strains / higher temperatures!Copy numberUnknownGene/InsertGene/Insert nameSpCas9_H840A_KK-MMLV-RT_co-P2A-PAC_dTKSpeciesSyntheticInsert Size (bp)8055PromoterCAGTags / Fusion ProteinsBPSV40 NLS (N terminal on insert)BPSV40 NLS (C terminal on insert)c-Myc NLS (C terminal on insert)Cloning InformationCloning methodLigation Independent Cloning5′ sequencing primerGGGCAAGTCCAAGAAACTG
Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net
All-in-one prime editor piggyBac transposon with PemaxpEX-A2Backbone manufacturerEurofins GenomicsBackbone size w/o insert (bp)5120Total vector size (bp)13175Modifications to backboneaddition of 3' and 5' piggyBac repeats, addition of pU6-pegRNA(B12B) sequence, addition of CAG promoter, addition of bGH (pA)Vector typeMammalian Expression, CRISPR, Synthetic Biology ; piggyBac transposonSelectable markersPuromycin ; thymidine kinaseGrowth in BacteriaBacterial Resistance(s)Ampicillin, 100 μg/mLGrowth Temperature30°CGrowth Strain(s)NEB StableGrowth instructionsIt is highly recommened to use NEB Stable at 30°C. Cloning efficiency and stability of plasmid can be compromised when using other strains / higher temperatures!Copy numberUnknownGene/InsertGene/Insert nameSpCas9_H840A_KK-MMLV-RT_co-P2A-PAC_dTKSpeciesSyntheticInsert Size (bp)8055PromoterCAGTags / Fusion ProteinsBPSV40 NLS (N terminal on insert)BPSV40 NLS (C terminal on insert)c-Myc NLS (C terminal on insert)Cloning InformationCloning methodLigation Independent Cloning5′ sequencing primerGGGCAAGTCCAAGAAACTG
Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net
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