TOM20MTS-mCherry-EGFP-Tet-On四环素诱导慢病毒双荧光表达质粒BioVector®载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

BioVector® NTCC® TOM20MTS-mCherry-EGFP-Tet-On四环素诱导慢病毒双荧光表达质粒BioVector®载体

Tet-On construct to induce expression of tandem eGFP-mCherry tagged TOM20MTS (mitochondria

Backbone size w/o insert (bp)7860
Vector typeMammalian Expression, Lentiviral
Selectable markersmCherry-eGFP
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Temperature37°C
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert
Gene/Insert nameTOM20MTS (MTS: mitochondria targeting signal)
SpeciesH. sapiens (human)
Insert Size (bp)1608
Entrez GeneTOMM20 (a.k.a. MAS20, MOM19, TOM20)
PromoterCMV promoter
Tag / Fusion Protein
mCherry-eGFP (C terminal on insert)
Cloning Information
Cloning methodLigation Independent Cloning
5′ sequencing primerCTCGTTTAGTGAACCGTCAGATCG
3′ sequencing primerCGTGAAGAATGTGCGAGACCC

Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net