Adult Mouse Hypothalamus Cell Line mHypoA-POMC/GFP-3成年小鼠下丘脑细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

BioVector® NTCC® Adult Mouse Hypothalamus Cell Line mHypoA-POMC/GFP-3成年小鼠下丘脑细胞株

Cell line name mHypoA-POMC/GFP-3
Accession CVCL_EP68
Resource Identification Initiative To cite this cell line use: mHypoA-POMC/GFP-3 (RRID:CVCL_EP68)
Comments Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
Derived from sampling site: Brain; hypothalamus.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: C57BL/6J-Tg(Pomc-EGFP)1Low/J.
Sex of cell Male
Age at sampling 8W
Category Transformed cell line

mHypoA-POMC/GFP-1-4 and mPitA-POMC/GFP-1

The mHypoA-POMC/GFP cell lines were generated using a proprietary platform technology enabling the creation of pools of immortalized hypothalamic neurons. These neurons were immortalized from four 8 week old male POMC-GFP mice (mouse 1-4) (strain C57BL/6J-Tg(Pomc-EGFP)1Low/J) hypothalamic primary cultures by retroviral transfer of SV40 T-Ag. These mice contain an eGFP transgene driven by the POMC promoter. The four immortalized hypothalamic pools were sorted on a cell sorting flow cytometer based on GFP fluorescence. Four cell lines representing the heterogeneous population of hypothalamic POMC neurons resulted: mHypoA-POMC/GFP lines 1–4.

These cells have been found to express an ever expanding array of neuropeptides, enzymatic markers and biologically active receptors. As such, these cells enable accurate in-vitro assays for use in the discovery, development and validation of new therapeutics targeted to central-nervous system diseases and disorders, including obesity, stress, and metabolic disorders, amongst others.

mPitA-POMC/GFP-1 was generated from a pool of immortalized pituitary neurons. These neurons were immortalized from 8 week old male POMC-GFP mouse (mouse 1) (strain C57BL/6J-Tg(Pomc-EGFP)1Low/J) pituitary primary cultures by retroviral transfer of SV40 T-Ag. The immortalized pituitary pool was sorted on a cell sorting flow cytometer based on GFP fluorescence as above. A cell line representing the heterogeneous population of POMC expressing pituitary neurons resulted: mPitA-POMC/GFP-1.

This pituitary cell line is easy to culture and has robust gene and protein expression. As such, this adult-derived pituitary cell line enables accurate assays for use in studies of progenitor cell characteristics and modulation, and the molecular analysis of hormone synthesis and secretion in differentiated cells.



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