AAVS1-Puro CAG-CLTX CD32a-tm CD3z CAR plasmid哺乳动物CAR-T基因编辑质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

BioVector® NTCC® AAVS1-Puro CAG-CLTX CD32a-tm CD3z CAR plasmid哺乳动物CAR-T基因编辑质粒载体

Donor plasmid for CLTX T-CAR expression in human cells

Backbone size w/o insert (bp)10225
Total vector size (bp)11513
Vector typeMammalian Expression, CRISPR, TALEN, Synthetic Biology
Selectable markersPuromycin
GROWTH IN BACTERIA
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Temperature37°C
Growth Strain(s)NEB Stable
Copy numberHigh Copy
GENE/INSERT
Gene/Insert nameSynthetic CLTX CD32a-tm CD3z CAR
SpeciesH. sapiens (human), Synthetic
Insert Size (bp)1288
PromoterCAG
CLONING INFORMATION
Cloning methodRestriction Enzyme
5′ cloning siteSalI (not destroyed)
3′ cloning siteMluI (not destroyed)
5′ sequencing primergctaaccatgttcatgccttc

Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net