HEK293-SpCas9 Stable Cell Line胚胎肾人细胞株 HEK293 从 AAVS1 位点稳定表达 SpCas9-HF1-NTCC细胞库

Cell Line Name：NTCC® HEK293-SpCas9 Stable Cell Line
Human cell line HEK293 stably expressing SpCas9-HF1 from AAVS1 site
人细胞株 HEK293 从 AAVS1 位点稳定表达 SpCas9-HF1胚胎肾
Selection Marker筛选标记：Hygro
Cat#货号:NTCC®-SL553
Size/Quantity数量: 1 Flask / 2 Vials
Biosafety Level生物安全级别: 1
Shipping Info运输方式: RT/Dry Ice
Storage储存方式: RT/Liquid Nitrogen
Description描述:BioVector NTCC Inc.’s stable cell lines constitutively expressing the CRISPR Cas9 nuclease enable you to carry out CRISPR genome editing applications with high efficiency. The NTCC® Cas9 stable cell lines are available pre-made in many human, mouse, and rat cell line backgrounds. The S. pyogenes CRISPR Cas9 nuclease is stably integrated into either the human AAVS1 or mouse ROSA26 “Safe Harbor” sites, or randomly via lentiviral-mediated transduction. The CRISPR Cas9 nuclease, in combination with single guide RNAs (sgRNAs), is widely used to create targeted genomic modifications, from gene knockouts, mutagenesis, fusion tagging, and more, in eukaryotic cells and animal models. Stable Cas9 integration ensures robust and consistent expression of the CRISPR Cas9 nuclease. Advantages Stable Cas9 integration minimizes the need for co-transfection or co-transduction of sgRNAs, ideal for high-throughput sgRNA applications. Single clone isolation provides consistent, high-level Cas9 expression in a uniform genetic background. Cas9 functionally validated using the NTCC® T7 Endonuclease I assay. Compatible with BioVector NTCC Inc. NTCC® sgRNA clones, sgRNA libraries, and donor clones.
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Other Collection No.其他编号:
Cell Type细胞类型: Embryonic kidney
Source组织来源:
Specification规格:
Cell Images细胞图片:
Complete Growth Medium完全培养基: NTCC® HEK293-SpCas9 Stable Cell Line Complete Medium
Subculturing传代方法:
Cryopreservation冻存方法:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: RT/Liquid nitrogen vapor phase
Culture Conditions培养条件:Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile鉴定数据:
References参考文献:

Supplier供应商:
BioVector质粒载体菌株细胞蛋白抗体基因保藏中心
NTCC典型培养物保藏中心
Tel电话：400-800-2947
Email：Biovector@163.com
http://www.biovector.net