S2-DGRC果蝇滋养细胞株Drosophila feeder cells -BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心
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- 货 号:NTCC®-S2-DGRC
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Synonyms: SL2 , Schneider's 2 , Schneider's line 2
Donor: Cherbas Lab
FlyBase Report:FBtc0000006
Cellosaurus Report:CVCL_TZ72
Features:
authenticated
Categories:
Dm Embryonic
This is the line which we formerly referred to as simply S2. We have changed its name in an effort to reduce the confusion in the literature about multiple isolates of the original Schneider's line 2.
Recommended Medium |
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Species | melanogaster |
Parent Line | isolate of Schneider's line 2 |
Primary Reference | FBrf0024118 |
Lab Of Origin | Schneider |
Stage Source | late embryonic stage |
Tissue Source | embryo |
Source | Strain: Oregon R |
Doubling Time |
All times are approximate (hrs, 25° unless otherwise noted). |
- Cell MaintenanceMaintain S2 cell line between 2.0 x 10e6 cells/mL - 1.0 x 10e7 cells/mL.
- HistoryMade from Oregon R embryos on the verge of hatching. This isolate was a gift from Cheeptip Benyajati to the Cherbas lab, ca. 1980.
- SexMale, by criterion of MSL complex assembly
- NoteMSL complex assembles on the X chromosome, implying a male character (Copps et al., 1998).
- S2 stocks free from mad cow diseaseOrders for S2 are normally filled from stocks of cells grown and frozen in medium containing recent lots of fetal calf serum of United States origin. We have a small stock of S2 cells from ampoules frozen prior to 2004 (the first known appearance of mad cow disease in the U.S.), then thawed and expanded in medium containing Australian serum (free from mad cow disease), and refrozen in a serum-free freezing medium. This stock is intended for users who intend to use the cells for pharmaceutical purposes, and who are legally required to use cells that have no possibility of contamination from mad cow disease.
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