AG12120不锈钢|顺序：CETARTIODACTYLA 主动脉，胸内皮细胞Cell Line细胞株-BioVector NTCC细胞库

Name细胞名称：NTCC® AG12120 SUS | ORDER: CETARTIODACTYLA Aorta, thoracic Cell Line
不锈钢|目：鲸齿兽细胞株
Description描述：Yorkshire breed. The thoracic aorta was removed immediately post-mortem on 3/12/92. The culture was initiated using cells released from the lumen of the vessel by collagenase digestion. The cell morphology is polygonal. The culture tested positive for the endothelial cell-specific von Willebrand factor and negative for alpha-smooth muscle actin (immunofluorescence assays). The culture was grown on gelatin-coated flasks and the medium was supplemented with ECGS (0.02 mg/ml) and heparin (0.05 mg/ml). Culture medium: HF12 with 10% plasma-derived donor horse serum with supplements. The HF12 medium used should be endotoxin-free. Other cultures from this pig are AG12077 and AG12119. The karyotype is 38,XX with 2% of the cells examined showing random chromosome loss. The legacy karyotype description shown in this Remark may not be representative of the current available product.
Category分类：Endothelial
ID编号:NTCC®-AG12120
Size/Quantity数量: 1 Flask/2 Vials
Biosafety Level生物安全级别:1
Shipping Info运输方式: RT/Dry Ice
Storage储存方式: RT/Liquid Nitrogen
Race:
Age年龄:10 WK
Gender性别:Female
Cell Type细胞类型: SUS | ORDER: CETARTIODACTYLA
Affected State:No
Product:Endothelial
Gene:
Mutations突变:
Ethnicity:YORKSHIRE
Family:1446
Relationship:proband
Karyotype:
Tissue Type组织来源:Artery
Complete Growth Medium完全培养基: BioVector® Ham's F12 with 2mM L-glutamine or equivalent Complete Medium
Subculturing传代方法:
Cryopreservation冻存方法:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions培养条件:Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile鉴定数据:
References参考文献:

Supplier供应商:BioVector质粒载体菌株细胞蛋白抗体基因保藏中心
NTCC典型培养物保藏中心
Tel电话：400-800-2947
Email：Biovector@163.com
http://www.biovector.net