AG11136明显健康的个体乳房类器官上皮Cell Line细胞株-BioVector NTCC细胞库

Name细胞名称：NTCC® AG11136 APPARENTLY HEALTHY INDIVIDUAL Breast organoid Cell Line
明显健康的个人细胞株
Description描述：Tissue was obtained in 1981 during subcutaneous mastectomy of breast contralateral to breast removed for tumor; pathologic diagnosis was mild fibrocystic disease. Mammary epithelial clumps or organoids were isolated by tissue dissection followed by partial enzymatic digestion with collagenase and hyaluronidase. Epithelial organoids were separated from the digested stromal elements by filtration and stored in liquid nitrogen. The culture was initiated 2/85 from thawed organoids by explantation in serum-free MCDB 170 plus supplements. Serial subcultivation resulted in the selection of a population of proliferative epithelial cells. Culture submitted as line 210C/AB470 at passage 5, PDL 22 (estimated by submitter), and has been frozen at the Repository at PDL 30. Culture is grown in serumfree medium. THIS SAMPLE WILL ONLY BE SHIPPED FROZEN
Category分类：Epithelial
ID编号:NTCC®-AG11136
Size/Quantity数量: 1 Flask/2 Vials
Biosafety Level生物安全级别:1
Shipping Info运输方式: RT/Dry Ice
Storage储存方式: RT/Liquid Nitrogen
Race: White
Age年龄:42 YR
Gender性别:Female
Cell Type细胞类型: APPARENTLY HEALTHY INDIVIDUAL
Affected State:No
Product:Epithelial
Gene:
Mutations突变:
Ethnicity:
Family:
Relationship:proband
Karyotype:
Tissue Type组织来源:Breast
Complete Growth Medium完全培养基: BioVector® MEGM-mammary epithelial cell medium plus supplements Complete Medium
Subculturing传代方法:
Cryopreservation冻存方法:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions培养条件:Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile鉴定数据:
References参考文献:

Supplier供应商:BioVector质粒载体菌株细胞蛋白抗体基因保藏中心
NTCC典型培养物保藏中心
Tel电话：400-800-2947
Email：Biovector@163.com
http://www.biovector.net