AG06814-MWI-38 - 正常人胎儿肺成纤维细胞 肺，肺成纤维细胞Cell Line细胞株-BioVector NTCC细胞库

Name细胞名称：NTCC® AG06814-M WI-38 - NORMAL HUMAN FETAL LUNG FIBROBLAST Lung, Lung Cell Line
WI-38 - 正常人胎儿肺成纤维细胞细胞株
Description描述：The WI-38 cell line was developed in July 1962 from lung tissue taken from a therapeutically aborted fetus of about 3 months gestational age. Cells released by trypsin digestion of the lung tissue were used for the primary culture. The cell morphology is fibroblast-like. The karyotype is 46,XX; normal diploid female. A maximum lifespan of 50 population doublings for this culture was obtained at the Repository. A thymidine labelling index of 86% was obtained after recovery. G6PD is isoenzyme type B. This culture of WI-38 is an expansion from passage 9 frozen cells obtained from the submitter. The legacy karyotype description shown in this Remark may not be representative of the current available product.
Category分类：Fibroblast
ID编号:NTCC®-AG06814-M
Size/Quantity数量: 1 Flask/2 Vials
Biosafety Level生物安全级别:1
Shipping Info运输方式: RT/Dry Ice
Storage储存方式: RT/Liquid Nitrogen
Race: White
Age年龄:12 FW
Gender性别:Female
Cell Type细胞类型: WI-38 - NORMAL HUMAN FETAL LUNG FIBROBLAST
Affected State:No
Product:Fibroblast
Gene:
Mutations突变:
Ethnicity:
Family:1053
Relationship:proband
Karyotype:46,XX
Tissue Type组织来源:Lung
Complete Growth Medium完全培养基: BioVector® Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent Complete Medium
Subculturing传代方法:
Cryopreservation冻存方法:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions培养条件:Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile鉴定数据:
References参考文献:

Supplier供应商:BioVector质粒载体菌株细胞蛋白抗体基因保藏中心
NTCC典型培养物保藏中心
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