AG11721 SUS | ORDER: CETARTIODACTYLA Aorta, thoracic Cell Line NTCC®细胞株-BioVector NTCC细胞库

Name细胞名称：NTCC® AG11721 SUS | ORDER: CETARTIODACTYLA Aorta, thoracic Cell Line
不锈钢|目：鲸齿兽细胞株
Description描述：Yorkshire breed. The thoracic aorta was removed immediately post-mortem on 10/21/91. Strips of the medial layer were removed, minced, and used as explants to initiate the culture. Cell morphology is fibroblast-like. The karyotype is 38,XX with 6% of the cells examined showing random chromosome loss. The culture tested positive for alpha-smooth muscle actin and negative for the endothelial cell-specific von Willebrand factor (immunofluorescence assays). Culture medium: HF12 15U. The HF12 medium used should be endotoxin-free. For lifespan determination this cell line was cultured on gelatin-coated flasks. Culture grows slowly. The legacy karyotype description shown in this Remark may not be representative of the current available product.
Category分类：Smooth muscle
ID编号:NTCC®-AG11721
Size/Quantity数量: 1 Flask/2 Vials
Biosafety Level生物安全级别:1
Shipping Info运输方式: RT/Dry Ice
Storage储存方式: RT/Liquid Nitrogen
Race:
Age年龄:2 MO
Gender性别:Female
Cell Type细胞类型: SUS | ORDER: CETARTIODACTYLA
Affected State:No
Product:Smooth muscle
Gene:
Mutations突变:
Ethnicity:YORKSHIRE
Family:
Relationship:proband
Karyotype:38,XX
Tissue Type组织来源:Artery
Complete Growth Medium完全培养基: BioVector® Endotoxin-Free Ham's F12 Medium with 2mM L-glutamine or equivalent Complete Medium
Subculturing传代方法:
Cryopreservation冻存方法:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions培养条件:Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile鉴定数据:
References参考文献:

Supplier供应商:BioVector质粒载体菌株细胞蛋白抗体基因保藏中心
NTCC典型培养物保藏中心
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