GM04339 CYSTIC FIBROSIS; CF | CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR; CFTR Cell Line NTCC®细胞株-BioVector NTCC细胞库

Name细胞名称：NTCC® GM04339 CYSTIC FIBROSIS; CF | CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR; CFTR Cell Line
囊性纤维化; CF |囊性纤维化跨膜电导调节器； CFTR细胞株
Description描述：Advanced chronic pulmonary disease; no pancreatic involvement; elevated sweat electrolytes; a sib died at age 4 with meconium ileus; defective fibroblast cAMP-stimulated Cl- channel; donor subject is a compound heterozygote: one allele carries the deltaF508 deletion mutation (PHE508DEL) and a second allele carries a G-to-A substitution at nucleotide 386 (386G>A) which converts the gly-85 codon (GGA) to a glu (GAA), resulting in a missense mutation in exon 3 in the CFTR gene [GLY85GLU (G85E)]
Category分类：Fibroblast
ID编号:NTCC®-GM04339
Size/Quantity数量: 1 Flask/2 Vials
Biosafety Level生物安全级别:1
Shipping Info运输方式: RT/Dry Ice
Storage储存方式: RT/Liquid Nitrogen
Race: White
Age年龄:24 YR
Gender性别:Male
Cell Type细胞类型: CYSTIC FIBROSIS; CF | CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR; CFTR
Affected State:Yes
Product:Fibroblast
Gene:CFTR
Mutations突变:GLY85GLU PHE508DEL
Ethnicity:
Family:706
Relationship:proband
Karyotype:
Tissue Type组织来源:
Complete Growth Medium完全培养基: BioVector® Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent Complete Medium
Subculturing传代方法:
Cryopreservation冻存方法:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions培养条件:Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile鉴定数据:
References参考文献:

Supplier供应商:BioVector质粒载体菌株细胞蛋白抗体基因保藏中心
NTCC典型培养物保藏中心
Tel电话：400-800-2947
Email：Biovector@163.com
http://www.biovector.net