GM04250 RETINOBLASTOMA, SPORADIC Skin, Arm Cell Line NTCC®细胞株-BioVector NTCC细胞库

Name细胞名称：NTCC® GM04250 RETINOBLASTOMA, SPORADIC Skin, Arm Cell Line
散发性视网膜母细胞瘤细胞株
Description描述：Clinically affected; diagnosed at 3 months of age with bilateral retinoblastoma; poor weight gain, weak sucking, cyanosis, and abnormal eye movement; slight normochromic anemia; developmental retardation; gross ophthalmological exam revealed leukocoria and sluggish pupillary light response in right eye; detailed exam of the right fundus showed a solid tumor with total retinal detachment, and the left fundus showed two elevated tumor masses; histological examination of the enucleated right eye revealed a well-differentiated retinoblastoma with focal necrosis and numerous rosette formation; procedures include: enucleation of right eye, transscleral cryocoagulation of the left eye, photocoagulation, and radiation; translocation of the long arm of chromosome 13 to the short arm of the X chromosome, karyotype: 46,XX,t(X;13)(p22; q13) in leukocytes; translocated X found to be preferentially inactivated in skin fibroblasts with the inactivation spreading over the translocated chromosome 13; chromosome studies of the parents and sibling revealed no abnormalities; erythrocyte esterase D type 1-1 identified in the proband, sibling and both parents.
Category分类：Fibroblast
ID编号:NTCC®-GM04250
Size/Quantity数量: 1 Flask/2 Vials
Biosafety Level生物安全级别:1
Shipping Info运输方式: RT/Dry Ice
Storage储存方式: RT/Liquid Nitrogen
Race: Asian
Age年龄:2 YR
Gender性别:Female
Cell Type细胞类型: RETINOBLASTOMA, SPORADIC
Affected State:Yes
Product:Fibroblast
Gene:
Mutations突变:
Ethnicity:JAPANESE
Family:
Relationship:proband
Karyotype:arr 2q13(110212011-110519397)x3
Tissue Type组织来源:Skin
Complete Growth Medium完全培养基: BioVector® Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent Complete Medium
Subculturing传代方法:
Cryopreservation冻存方法:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions培养条件:Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile鉴定数据:
References参考文献:

Supplier供应商:BioVector质粒载体菌株细胞蛋白抗体基因保藏中心
NTCC典型培养物保藏中心
Tel电话：400-800-2947
Email：Biovector@163.com
http://www.biovector.net