GM16344 FACIOSCAPULOHUMERAL MUSCULAR DYSTROPHY 1; FSHD1 B-Lymphocyte Cell Line NTCC®细胞株-BioVector NTCC细胞库

Name细胞名称：NTCC® GM16344 FACIOSCAPULOHUMERAL MUSCULAR DYSTROPHY 1; FSHD1 B-Lymphocyte Cell Line
面肩肱型肌营养不良症 1； FSHD1细胞株
Description描述：Unaffected; D4Z4 fragment size was determined using Southern Blot analysis with probe p13E-11, which is localized immediately proximal to D4Z4, after double digestion of genomic DNA with EcoRI and BlnI restriction enzymes. The D4Z4 microsatellite is not contracted in this individual. The permissive haplotype 4qA carries polyadenylation signal (PAS) as determined using the primers and protocol described by Lemmers R.J.F. et al (Science, 2010). The 4qA, 4qB and chromosome 10q haplotype structures were determined by 4A/4A-L/4B subtelomere haplotyping, SSLP haplotyping, and 4qSTR analysis as described by Lemmers R.J.F. et al (Am J Hum Genet., 2010 and Science, 2010), Snider L. et al (PLoS Genetics, 2010), and Barat-Houari M. et al (Euro J Hum Genet., 2010) and are as follows 4A161/4*162, 10A166/10A166; characterization of this line described by T.I. Jones et al, Neuromuscular Disorders (2016), http://dx.doi.org/doi:10.1016/j.nmd.2016.12.007; several affected family members; refer to catalog Family 1947 (Family 33 in the Jones publication).
Category分类：B-Lymphocyte
ID编号:NTCC®-GM16344
Size/Quantity数量: 1 Flask/2 Vials
Biosafety Level生物安全级别:1
Shipping Info运输方式: RT/Dry Ice
Storage储存方式: RT/Liquid Nitrogen
Race:
Age年龄:
Gender性别:Female
Cell Type细胞类型: FACIOSCAPULOHUMERAL MUSCULAR DYSTROPHY 1; FSHD1
Affected State:No
Product:LCL
Gene:
Mutations突变:
Ethnicity:
Family:1947
Relationship:in-law
Karyotype:
Tissue Type组织来源:Blood
Complete Growth Medium完全培养基: BioVector® Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent Complete Medium
Subculturing传代方法:
Cryopreservation冻存方法:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions培养条件:Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile鉴定数据:
References参考文献:

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