iSLK.219癌细胞株NTCC® Cancer cell line细胞株/完全培养基-BioVector NTCC典型培养物保藏中心

[Designation名称] NTCC® ID iSLK.219 Cell Line细胞株
BioVector NTCC Inc.

DR Wikidata; Q111733519
RX PubMed=21419799;
SY iSLK.219 clone #10
RX PubMed=22987579;
CC Problematic cell line: Contaminated. Shown to be a Caki-1 derivative (PubMed=22987579). The grand-parent cell line was originally thought to originate from a 28 year old male patient with HIV-1 negative Kaposi sarcoma.
CC Population: Caucasian.
CC Characteristics: rKSHV.219 includes a RFP-GFP-Puro construct that contains RFP under the control of the lytic PAN RNA promoter, GFP under the control of human EF1a promoter and PuroR expressed by the RSV promoter.
CC Characteristics: Transduced with pRetroX-Tet-On that contains a reverse tetracycline-controlled transactivator (rtTA), a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16 and with RetroX-Tight-Hyg-RTA.
CC Virology: Maintains latent rKSHV.219 as indicated by expression of constitutively expressed (eGFP) but not a lytic phase (RFP) reporter gene. When exposed to either sodium butyrate or doxycycline, the cells are activated to lytic replication and express RFP and KSHV lytic genes and release large quantities of infectious virus (PubMed=21419799).
CC Virology: Infected with a recombinant human herpesvirus 8 (HHV-8/KSHV) rKSHV.219.
CC Sequence variation: Mutation; HGNC; 9884; RB1; Simple; p.Leu98Pro (c.293T>C); ClinVar=VCV001502192; Zygosity=Heterozygous (from parent cell line).
CC Transfected with: UniProtKB; Q9U6Y8; Discosoma sp. red fluorescent protein drFP583 (DsRed, RFP).
CC Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
[Organism物种来源]
[Disease疾病种类]
[Accession编号] NTCC®-AC CVCL_B6YV
[Comments描述]
[Reference参考文献]
[Category分类]
[Date保藏时间]
[Synonyms别名]
[Hierarchy]
培养方法：
Medium培养基：NTCC® ID iSLK.219 Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Website网址：www.biovector.net