HepG2/8F_HS癌细胞株NTCC® Cancer cell line细胞株/完全培养基-BioVector NTCC典型培养物保藏中心

[Designation名称] NTCC® ID HepG2/8F_HS Cell Line细胞株
BioVector NTCC Inc.

RX PubMed=35406758;
CC Population: Caucasian.
DR RCB; RCB5677
CC Characteristics: Could be useful as a new cell source for pharmacological studies and constructing bioartificial liver systems. When heat-stimulated it expresses 8 liver-specific transcription factor genes and thus many liver functions are induced upon heat treatment (PubMed=35406758).
CC Characteristics: Transduced with a pQMSCV-pHSP70B-TRE-pCMVmin-EGFP-IRES-tTA construct. The heat-activated HSPA6/HSP70B' promoter drives bicistronic gene expression of EGFP and of the tetracycline-controlled transactivator (tTA), which is mediated by an internal ribosomal entry site (IRES). Then, the tTA protein binds to TRE and activates the cytomegalovirus minimum promoter (pCMVmin) upstream of EGFP, which in turn induces further expression of the EGFP and tTA genes, providing a transcriptional positive feedback loop (from parent cell line).
CC Characteristics: The amplified tTA promotes the expression of 8 liver-enriched transcription factor (LETF) genes that were transduced using two constructs: PB513B-1/TRE-HNF1A-FOXA2/HNF3B-CEBPA-CEBPB-CEBPG-EF1a-PuroR and PB513B-1/TRE-HNF1B-HNF4A-ONECUT1-EF1a-Bla (PubMed=35406758).
CC Sequence variation: Mutation; HGNC; 7989; NRAS; Simple; p.Gln61Leu (c.182A>T); ClinVar=VCV000375874; Zygosity=Heterozygous (from parent cell line).
CC Sequence variation: Mutation; HGNC; 11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
CC Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
CC Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC Transfected with: UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (with p.Met1_Arg360del).
CC Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
CC Transfected with: UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd).
[Organism物种来源]
[Disease疾病种类]
[Accession编号] NTCC®-AC CVCL_D3XF
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[Reference参考文献]
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[Date保藏时间]
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培养方法：
Medium培养基：NTCC® ID HepG2/8F_HS Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Website网址：www.biovector.net