HeLa-Difluo hLC3癌细胞株NTCC® Cancer cell line细胞株/完全培养基-BioVector NTCC典型培养物保藏中心

[Designation名称] NTCC® ID HeLa-Difluo hLC3 Cell Line细胞株
BioVector NTCC Inc.

WW https://www.invivogen.com/hela-difluo-hlc3
CC Population: African American.
DR Wikidata; Q107115322
CC Characteristics: Allows monitoring of autophagic flux in real time by detecting the appearance of dual red and green RFP:GFP:LC3 puncta or single red RFP:LC3 puncta by fluorescence microscopy. Early in autophagy, both RFP and GFP signals are detected. As the fusion of the autophagosomes with the lysosomes progresses, the GFP fluorescence diminishes, leaving only the RFP fluorescence visible. The percentages of RFP-GFP positive and of RFP positive cells can be determined and these values can be used to assess autophagic flux (InvivoGen).
CC Characteristics: Stably expresses a RFP-GFP-MAP1LC3B construct.
CC Transfected with: HGNC; 13352; MAP1LC3B.
CC Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
CC Transfected with: UniProtKB; Q9U6Y8; Discosoma sp. red fluorescent protein drFP583 (DsRed, RFP).
CC Transfected with: UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
CC Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
CC Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
DI NCIt; C27677; Human papillomavirus-related cervical adenocarcinoma
OX NCBI_TaxID=9606; ! Homo sapiens (Human)
[Organism物种来源]
[Disease疾病种类]
[Accession编号] NTCC®-AC CVCL_A8CA
[Comments描述]
[Reference参考文献]
[Category分类]
[Date保藏时间]
[Synonyms别名]
[Hierarchy]
培养方法：
Medium培养基：NTCC® ID HeLa-Difluo hLC3 Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Website网址：www.biovector.net