HeLa HaloTag-LC3/ATG3-Y209A-mCherry癌细胞株NTCC® Cancer cell line细胞株/完全培养基-BioVector NTCC典型培养物保藏中心

[Designation名称] NTCC® ID HeLa HaloTag-LC3/ATG3-Y209A-mCherry Cell Line细胞株
BioVector NTCC Inc.

RX DOI=10.1101/2023.07.17.549391;
CC Population: African American.
DR Wikidata; Q123031616
CC Characteristics: Stably expresses HaloTag-LC3 to measure autophagic flux. Also expresses an internally mCherry-tagged ATG3 with p.Tyr209Ala.
CC Knockout cell: Method=CRISPR/Cas9; HGNC; 20962; ATG3.
CC Transfected with: HGNC; 20962; ATG3 (With p.Tyr209Ala and with a mCherry reporter inserted between Glu-125 and Ile-126).
CC Transfected with: RGD; 621315; Map1lc3b.
CC Transfected with: UniProtKB; X5DSL3; Anaplasma marginale mCherry fluorescent protein.
CC Transfected with: UniProtKB; P0A3G2; Rhodococcus rhodochrous dhaA (mutated = HaloTag).
CC Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
CC Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
DI NCIt; C27677; Human papillomavirus-related cervical adenocarcinoma
[Organism物种来源]
[Disease疾病种类]
[Accession编号] NTCC®-AC CVCL_C8QF
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[Reference参考文献]
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[Date保藏时间]
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培养方法：
Medium培养基：NTCC® ID HeLa HaloTag-LC3/ATG3-Y209A-mCherry Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Website网址：www.biovector.net