HEK293T-CRISPRa转化细胞株NTCC® Transformed cell line细胞株/完全培养基-BioVector NTCC典型培养物保藏中心

[Designation名称] NTCC® ID HEK293T-CRISPRa Cell Line细胞株
BioVector NTCC Inc.

DR Wikidata; Q123031592
RX PubMed=34431042;
DR BioGRID_ORCS_Cell_line; 1656
CC Characteristics: Transduced with lentiviral vectors dCAS-VP64_Blast (EF1a-dCas9-VP64-Bsd-WPE) and MS2-P65-HSF1_Hygro (EF1a-MS2(N55K)-SV40(NLS)-RELA-HSF1-hygR-WPRE) (PubMed=34431042).
CC Transfected with: HGNC; 5224; HSF1.
CC Transfected with: MGI:103290; Rela (with p.Met1_Leu368 del).
CC Transfected with: UniProtKB; P03612; Escherichia phage MS2 coat protein (with p.Asn56Lys).
CC Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (with p.Asp10Ala and p.His840Ala = dCas9).
CC Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
CC Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC Transfected with: UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd).
CC Transformant: NCBI_TaxID; 28285; Adenovirus 5.
CC Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) [Note=tsA].
[Organism物种来源]
[Disease疾病种类]
[Accession编号] NTCC®-AC CVCL_C9DX
[Comments描述]
[Reference参考文献]
[Category分类]
[Date保藏时间]
[Synonyms别名]
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培养方法：
Medium培养基：NTCC® ID HEK293T-CRISPRa Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Website网址：www.biovector.net