HEK293-DR-GFP-RAD51B-9/B+转化细胞株NTCC® Transformed cell line细胞株/完全培养基-BioVector NTCC典型培养物保藏中心

[Designation名称] NTCC® ID HEK293-DR-GFP-RAD51B-9/B+ Cell Line细胞株
BioVector NTCC Inc.

DR DSMZCellDive; ACC-850
DR Wikidata; Q94095046
DR DSMZ; ACC-850
CC Characteristics: The parental RAD51B KO cell line is functionally reconstituted by retroviral transfection of RAD51B.
CC Characteristics: Transfected with the hprtDR-GFP reporter construct that targets the DR-GFP into the hprt locus and includes a dominant selectable puroR marker. The DR-GFP construct which contains a tandem repeat of two inactive genes coding for EGFP. After transfection with an expression vector coding for the I-SceI enzyme, recombination induced by the cleavage of the I-SceI site recreates a functional EGFP.
CC Characteristics: Transfected with the tetracycline transactivator (tTA) protein, a fusion of tetR with the activation domain of HSV-1 VP16.
CC Doubling time: ~24-36 hours (DSMZ=ACC-850).
CC Knockout cell: Method=CRISPR/Cas9; HGNC; 9822; RAD51B.
CC Transfected with: HGNC; 9822; RAD51B.
CC Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
CC Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC Transfected with: UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (with p.Met1_Arg360del).
CC Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
[Organism物种来源]
[Disease疾病种类]
[Accession编号] NTCC®-AC CVCL_XX06
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[Reference参考文献]
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[Date保藏时间]
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培养方法：
Medium培养基：NTCC® ID HEK293-DR-GFP-RAD51B-9/B+ Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Website网址：www.biovector.net