HEC-LTT条件永生化细胞株NTCC® Conditionally immortalized cell line细胞株/完全培养基-BioVector NTCC典型培养物保藏中心

[Designation名称] NTCC® ID HEC-LTT Cell Line细胞株
BioVector NTCC Inc.

DR Wikidata; Q107115253
RX PubMed=19705962;
SY HUVEC Uni-TAg/bi-hTert
RX PubMed=26345505;
CC Characteristics: Transfected with reverse tetracycline-controlled transactivator (rtTA), a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16.
CC Characteristics: In the presence of doxycycline, hTERT and SV40-TAg expression are activated, resulting in high cell proliferation and unlimited expansion. In the absence of the doxycycline, cells stop proliferating and can be maintained in a growth-arrested state that reflects the status of endothelial cells in vivo (PubMed=26345505).
CC Transfected with: HGNC; 11730; TERT.
CC Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
CC Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC Transfected with: UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (with p.Met1_Arg360del).
CC Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
CC Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
CC Derived from site: In situ; Umbilical vein; UBERON=UBERON_0002066.
[Organism物种来源]
[Disease疾病种类]
[Accession编号] NTCC®-AC CVCL_A7FW
[Comments描述]
[Reference参考文献]
[Category分类]
[Date保藏时间]
[Synonyms别名]
[Hierarchy]
培养方法：
Medium培养基：NTCC® ID HEC-LTT Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Website网址：www.biovector.net