pNOY3148, TrpE-RRN4 出芽酵母载体质粒BioVector NTCC质粒载体菌种细胞基因保藏中心Budding yeast vector

pNOY3148, TrpE-RRN4  【载体类型Vector Type】： 【插入基因Inserted Gene】： trpE-RPA12  【大肠杆菌筛选标记E.coli selection marker】： Amp  【酵母筛选标记S.pombe selection marker】： 【其他Comments】: BamHI site of pATH22 was cut, repaired and ligated (ΔBamHI-ATH22). 8 bp (CGGATCCG) of BamHI linker was inserted at SmaI site of ΔBamHI-ATH22. Then, the 0.6 kb BamHI-XhoI fragment containing RPA12 was inserted between BamHI and SalI sites of the above vector. BamHI site was created at the ScaI site of RPA12 gene by insertion of the 8 bp BamHI linker, Yas Nogi