pNOY3098 出芽酵母载体质粒BioVector NTCC质粒载体菌种细胞基因保藏中心Budding yeast vector

pNOY3098  【载体类型Vector Type】： 【插入基因Inserted Gene】： RPA190  【大肠杆菌筛选标记E.coli selection marker】： Amp  【酵母筛选标记S.pombe selection marker】： 【其他Comments】: XhoI/BglII fragment containing RPA190 gene cloned into XhoI/SalI sites of pNOY3095. Done by cutting pNOY16 DNA with BglII and filling in with Klenow with only dGTP and dATP. Then cut with XhoI and RPA190 fragment isolated on Sea Plaque Agarose. Vector pNOY3095 cut with SalI then treated with Klenow with only dCTP and dTTP, then cut with XhoI and ligated with RPA190 fragment, Mike Wittekind