pNOY403, DLp759 出芽酵母载体质粒BioVector NTCC质粒载体菌种细胞基因保藏中心Budding yeast vector

pNOY403, DLp759  【载体类型Vector Type】：YCp  【插入基因Inserted Gene】： 3HAI-RRN7-His tag  【大肠杆菌筛选标记E.coli selection marker】： Amp  【酵母筛选标记S.pombe selection marker】： TRP1  【其他Comments】: Replacing the 433 bp BglII-SacI fragment of pNOY303 by the 468 bp BglII-SacI fragment from DLp760 (DLp760 was made by insertion of the carboxyterminal end of HAI-RRN7 and His tag by site directed mutagenesis, using plasmid pNOY252 (HAI-RRN7/pRS314) and oligo RRN7-E (antiparallele of 5' gcctgtctgcataggatgaatAGCAGCCATCATCATCATCATCACAGCAGCTGAGCtgaCTCCTTTTAAATTATTCTCG 3', adding S.S. (H)6 SS just before the STOP codon), CEN6, ARS4, TRP1, (3HAI)-RRN7-His tag, D. Lalo