pNOY373, pHW71'xN 出芽酵母载体质粒BioVector NTCC质粒载体菌种细胞基因保藏中心Budding yeast vector

pNOY373, pHW71'xN  【载体类型Vector Type】：YEp  【插入基因Inserted Gene】： RDN1  【大肠杆菌筛选标记E.coli selection marker】： Amp  【酵母筛选标记S.pombe selection marker】： LEU2  【其他Comments】: A derivtive of pHW71 (=BamHI fragment of pRDN-Wt into BamHI site of YEp351 (2_m LEU2)) except (1) the promoter region (SalI site from the vector and NdeI site at +724) was replaced by the -210 SmaI to +724 NdeI from pNOY3106 by blunt-ended SalI-NdeI sites of pHW71, (2) The enhancer is now flanked by the newly introduced XhoI (5'end of R1) and NotI (3' HpaI) via site directed mutagenesis. ~14.8 kb