pNOY329, 230T-1 出芽酵母载体质粒BioVector NTCC质粒载体菌种细胞基因保藏中心Budding yeast vector

pNOY329, 230T-1  【载体类型Vector Type】：YCp  【插入基因Inserted Gene】： RRN5-ADC1ter  【大肠杆菌筛选标记E.coli selection marker】： Amp  【酵母筛选标记S.pombe selection marker】： LEU2  【其他Comments】: 1.56 kb RRN5 gene was amplified by PCR using pNOY230 creating SalI site at the 5' end and NotI site at the 3' end. The amplified region is +1~+1561 on Emmanuelle's map. The NotI-SalI RRN5 was cloned between NotI and SalI of pNOY328, Ologo NOGI-18 (37mer) 5' AAATGCGGCCGCCTTTGGATAACCATTTTAGCAACT 3', Ologo NOGI-19 (28mer) 5' AAGTCGACGAATTCTGCTTTTGGAAGAC 3'. NOGI-18 hybridizes +1~+20 of RRN5, and NOGI-19 hybridizes +1561~+1540 of RRN5 on Emmanuelle's map, 8.3 kb, Y. Nogi