pNOY206, pZ21-28 出芽酵母载体质粒BioVector NTCC质粒载体菌种细胞基因保藏中心Budding yeast vector

pNOY206, pZ21-28  【载体类型Vector Type】：YEp  【插入基因Inserted Gene】： RRN3  【大肠杆菌筛选标记E.coli selection marker】： Amp  【酵母筛选标记S.pombe selection marker】： LEU2  【其他Comments】: 2.1 kb PvuII (9 bp from ATG) -XbaI fragment contain RRN3 was inserted between BamHI and XbaI site of YEp315 (pNOY25). YEp315 was cut with BamHI, filled in by Klenow then cut with XbaI. The PvuII from the insert ligated to the Blunt-ended BamHI from the vector regenerated the BamHI site, 7.7 kb, Y. Nogi