pNOY158, pY21-30 出芽酵母载体质粒BioVector NTCC质粒载体菌种细胞基因保藏中心Budding yeast vector

pNOY158, pY21-30  【载体类型Vector Type】：YEp  【插入基因Inserted Gene】： rpa12:: LEU2  【大肠杆菌筛选标记E.coli selection marker】： Amp  【酵母筛选标记S.pombe selection marker】： LEU2  【其他Comments】: LEU2 gene (2.2 kb) flanked by BamHI and PstI replaced ScaI-PstI region of RPA12 to disrupt RPA12. ScaI site was converted to BamHI site. This fusion fragment was cloned to between HindIII and SmaI sites of YEp351. To disrupt RPA12, cut pNOY158 with HindIII and XhoI to get the 3.2 kb fragment transform into yeast, Y. Nogi