pNOY147, pYA303 出芽酵母载体质粒BioVector NTCC质粒载体菌种细胞基因保藏中心Budding yeast vector

pNOY147, pYA303  【载体类型Vector Type】：YEp  【插入基因Inserted Gene】： 【大肠杆菌筛选标记E.coli selection marker】： Amp  【酵母筛选标记S.pombe selection marker】： HIS3  【其他Comments】: Multicopy vector (2_-ori) with HIS3+. 2_ DNA flagment (EcoRI-EcoRI, 2241 bp) cut out from YEp24 (pNOY77) was ligated into pRS303 (pNOY66) EcoRI site in multicloning site. Some cloning sites (KpnI, HindIII, EcoRI, PstI, SpeI, XbaI, BstXI) are not available as single cutting sites, [pUC-ori (E. coli), 2_-Ori (yeast), HIS3+ (yeast), AmpR (E. coli)], R. Yano