pNOY133, p103KS 出芽酵母载体质粒BioVector NTCC质粒载体菌种细胞基因保藏中心Budding yeast vector

pNOY133, p103KS  【载体类型Vector Type】：YCp  【插入基因Inserted Gene】： upstream and downstream of NSM1 (SRP1) 【大肠杆菌筛选标记E.coli selection marker】： Amp  【酵母筛选标记S.pombe selection marker】： URA3  【其他Comments】: The KpnI-HpaI (~2 kb) fragment upstream of NSM1 gene and the BglII-SacI (0.8 kb) fragment downstream of NSM1 gene were cloned into KpnI-EcoRV and BamHI-SacI sites of pRS316, respectively. NSM1 gene was cut out from pSRP103 (pNOY119). This plasmid is for cloning of wild type gene of NSM1 with transformation after cutting by SmaI. vector::pRS316 (pNOY73) (URA+, AmpR), Ryoji Yano