pNOY16 出芽酵母载体质粒BioVector NTCC质粒载体菌种细胞基因保藏中心Budding yeast vector

pNOY16  【载体类型Vector Type】：YCp  【插入基因Inserted Gene】： RPA190  【大肠杆菌筛选标记E.coli selection marker】： Amp  【酵母筛选标记S.pombe selection marker】： TRP1  【其他Comments】: Similar to pNOY3079 except has more RPA190 5' flanking DNA made by putting SAL TAB linkers pdCGTCGA (see Pharmacia) onto 6.7 kb Cla fragment which contains 5' end of RPA190 + 5' flanking DNA. This was cut with NcoI and ligated with Sal/NcoI cut pNOY3079. Plasmid has ~11.5 kb of yeast DNA containing intact RPA190 gene plus 5' & 3' flank.; Mike Wittekind