TOp2528 出芽酵母载体质粒BioVector NTCC质粒载体菌种细胞基因保藏中心Budding yeast vector

TOp2528  【载体类型Vector Type】：YCp  【插入基因Inserted Gene】： bul1  【大肠杆菌筛选标记E.coli selection marker】： Amp  【酵母筛选标记S.pombe selection marker】： URA3  【其他Comments】: (I) PCR(bul1seq1, BUL1-C942S-r, TOp2518) Klenow, PCR (BUL1-C942S, BUL1-2, TOp2518) Klenow, , Klenow, (II) PCR (bul1seq1, BUL1-2, (I)PCR product 2 pecies), ClaII, SalI (1050bp fragment), L. TOp2468 (ClaI, SalI large fragment), around 3840 bp C→S 3 sites were changed