TOp2525 出芽酵母载体质粒BioVector NTCC质粒载体菌种细胞基因保藏中心Budding yeast vector

TOp2525  【载体类型Vector Type】：YEp  【插入基因Inserted Gene】： BUL1-FPRI  【大肠杆菌筛选标记E.coli selection marker】： Amp  【酵母筛选标记S.pombe selection marker】： HIS3  【其他Comments】: (I) PCR(BUL1-1, BUL1/FPR1-r, W303D) Klenow, PCR (BUL1/FPR1, FPR1-1, W303D) Klenow, , (II) PCR (BUL1-1, FPR1-1, (I)PCR product 2 pecies), NotII, NdeI (cut out 1940bp and 520bp) →520bp was digested with XhoI → L. pYO323 (NotI, XhoI) , IIPCR NotI, NdeI 1940bp- IIPCR NdeI-XhoI 520bp-pYO323, BUL1-FPRI-pYO323