U2OS#18-RAD51C-15/C+ Cancer cell line NTCC®细胞株/完全培养基-BioVector NTCC典型培养物保藏中心

[Designation名称] NTCC® U2OS#18-RAD51C-15/C+ Cell Line细胞株
BioVector NTCC Inc.

Cross-references: DSMZCellDive; ACC-839
Cross-references: Wikidata; Q98133780
Cross-references: DSMZ; ACC-839
Comments: Population: Caucasian.
Comments: Characteristics: The parental RAD51C KO cell line is functionally reconstituted by retroviral transfection of RAD51C.
Comments: Characteristics: Contains a 'nested intron' reporter construct HRsub that allows to study sister chromatid recombination (SCR) by positive selection of 'long tract' gene conversion events between sister chromatids.
Comments: Doubling time: ~25-40 hours (DSMZ=ACC-839).
Comments: Knockout cell: Method=CRISPR/Cas9; HGNC; 9820; RAD51C.
Comments: Sequence variation: Mutation; HGNC; 9277; PPM1D; Simple; p.Arg458Ter (c.1372C>T); ClinVar=VCV002445214; Zygosity=Heterozygous (from parent cell line).
Comments: Transfected with: HGNC; 9820; RAD51C.
Comments: Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
Comments: Transfected with: UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd).
Comments: Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
[Organism物种来源]
[Disease疾病种类]
[Accession编号] NTCC® Accession: NTCC_VR81
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[Reference参考文献]
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培养方法：
Medium培养基：NTCC® Specific Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Website网址：www.biovector.net