U2OS MPX-Nomad Cancer cell line NTCC®细胞株/完全培养基-BioVector NTCC典型培养物保藏中心

[Designation名称] NTCC® U2OS MPX-Nomad Cell Line细胞株
BioVector NTCC Inc.

Cross-references: Wikidata; Q98133757
References identifiers: PubMed=29412765;
Synonyms: MPXNomad; MultiPleXed-Nomad U2OS
Comments: Population: Caucasian.
Comments: Characteristics: The green calcium-Nomad biosensor consists of a membrane localization peptide derived from interleukin-2 receptor alpha chain, a TurboGFP fluorescent protein, a calmodulin-binding peptide fused to a KDEL signal.
Comments: Characteristics: The red cAMP-Nomad biosensor consists of a membrane localization peptide derived from interleukin-2 receptor alpha chain, a TurboFP650 fluorescent protein, a Ht31 peptide fused to a KDEL signal.
Comments: Sequence variation: Mutation; HGNC; 9277; PPM1D; Simple; p.Arg458Ter (c.1372C>T); ClinVar=VCV002445214; Zygosity=Heterozygous (from parent cell line).
Comments: Transfected with: UniProtKB; J9PBR7; Entacmaea quadricolor far-red fluorescent protein eqFP650.
Comments: Transfected with: UniProtKB; Q6WV12; Pontellina plumata GFP2 (with p.Lys5Glu, p.Val117Met, p.Val149Asp, p.Val151Asp, p.Ala155Thr, p.Phe168Ser and p.Leu200Asp = TurboGFP).
Comments: Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Comments: Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
Comments: Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979.
Diseases: NCIt; C9145; Osteosarcoma
[Organism物种来源]
[Disease疾病种类]
[Accession编号] NTCC® Accession: NTCC_YK69
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[Date保藏时间]
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培养方法：
Medium培养基：NTCC® Specific Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Website网址：www.biovector.net