SAM iPSC clone 1 Induced pluripotent stem cell NTCC®细胞株/完全培养基-BioVector NTCC典型培养物保藏中心

[Designation名称] NTCC® SAM iPSC clone 1 Cell Line细胞株
BioVector NTCC Inc.

References identifiers: PubMed=27934604;
Comments: Characteristics: Stably expresses dCas9-VP64, a nuclease-deficient mutant of SpCas9 (dCas9) under the control of a promoter containing 4 copies of the activating domain of HSV-1 VP16 (termed VP64) and EF1a-MS2-P65-HSF1-2A-HygR where the coat protein of MS2 fused to the transactivation domain of p65(RELA) and of heat shock factor 1 (HSF1) (PubMed=27934604).
Cross-references: Wikidata; Q105510959
Comments: Transfected with: HGNC; 5224; HSF1.
Comments: Transfected with: HGNC; 9955; RELA.
Comments: Transfected with: UniProtKB; P03612; Escherichia phage MS2 capsid protein (with p.Asn56Lys).
Comments: Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (with p.Asp10Ala and p.His840Ala = dCas9).
Comments: Transfected with: UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd).
Comments: Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Comments: Derived from site: In situ; Skin, dermis; UBERON=UBERON_0002067.
Comments: Cell type: Fibroblast of skin; CL=CL_0002620.
Species of origin: NCBI_TaxID=9606; ! Homo sapiens (Human)
Originate from same individual: NTCC_A4GY ! SAM iPSC clone 2
[Organism物种来源]
[Disease疾病种类]
[Accession编号] NTCC® Accession: NTCC_A4GX
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[Reference参考文献]
[Category分类]
[Date保藏时间]
[Synonyms别名]
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培养方法：
Medium培养基：NTCC® Specific Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Website网址：www.biovector.net