RUCDRi002-A-15 Induced pluripotent stem cell NTCC®细胞株/完全培养基-BioVector NTCC典型培养物保藏中心

[Designation名称] NTCC® RUCDRi002-A-15 Cell Line细胞株
BioVector NTCC Inc.

Cross-references: hPSCreg; RUCDRi002-A-15
Cross-references: Wikidata; Q107116790
Synonyms: TC-1133-CRISPRa2; hiPSC-CRISPRa2; UMGi147-A-15
References identifiers: PubMed=34481190;
Web pages: https:sfb1002.med.uni-goettingen.de/production/cellmodel/cell-line/view?tab=gen_mod&line=478
Comments: From: University Medical Center Goettingen; Goettingen; Germany.
Comments: Population: Hispanic.
Comments: Characteristics: Using CRISPR/Cas9 a dCas9-VPR-2A-tdTomato-WPRE construct containing dCas9 fused to heterotrimeric VPR transactivator consisting of VP64, p65, and RTA domains along with a tdTomato and a woodchuck hepatitis post-transcriptional regulatory element (WPRE) was introduced in the AAVS1 safe harbor locus (PubMed=34481190).
Comments: Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (with p.Asp10Ala and p.His840Ala = dCas9).
Comments: Transfected with: tdTomato, a bright red fluorescent protein derived from drFP583 (DsRed).
Comments: Derived from site: In situ; Umbilical cord blood; UBERON=UBERON_0012168.
Species of origin: NCBI_TaxID=9606; ! Homo sapiens (Human)
Hierarchy: NTCC_RH35 ! ND50038
[Organism物种来源]
[Disease疾病种类]
[Accession编号] NTCC® Accession: NTCC_A7LR
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[Reference参考文献]
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[Date保藏时间]
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培养方法：
Medium培养基：NTCC® Specific Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Website网址：www.biovector.net