K-12 HB101 BioVector NTCC质粒载体菌种细胞基因保藏中心

K-12 HB101

ChromosomalGenotype

F-mcrB mrr hsdS20(rB- mB-) recA13 leuB6 ara-14 proA2 lacY1 galK2 xyl-5 mtl-1rpsL20(SmR) glnV44 λ-

Description

Hybrid of E. coli K12 and E. coli B (but 98% K strain AB266 according toSmith et al.)

Host for pBR322 and many plasmids

Streptomycin resistant

References:

Boyer, H.W. and Roulland-Dussoix, D. (1969) J. Mol. Biol. 41, 459.

Smith, M., Lorow, D., and Jessee, J. (1989) FOCUS 11, 56 - pdf version fromInvitrogen

Lacks S and Greenberg JR (1977) J Mol Biol 114:153.

Recovery

1.Obtainan LB agar plate with the appropriate antibiotic.

2.Usinga sterile pipette tip, touch the bacteria growing within the punctured area ofthe stab culture. (A sterilized wire loop or sterile toothpick can be used inplace of a sterile pipette tip.)

3.Runthis tip lightly over a section of the plate, as shown in the figure, to createstreak #1.

4.Usinganother sterile pipette tip, pass through streak #1 and spread the bacteriaover a second section of the plate, to create streak #2.

5.Usinga third sterile pipette tip, pass through streak #2 and spread the bacteriaover the last section of the plate, to create streak #3.

6.Growovernight in a 37 ^o C incubator (unless a different growthtemperature is indicated on the plasmid datasheet).

7.Inthe morning, single colonies should be visible. If the bacterial growth is toodense, re-streak onto a new agar plate to obtain single colonies.

BioVector NTCC质粒载体菌种细胞基因保藏中心