KBM5-T315I细胞 BioVector NTCC质粒载体菌种细胞基因保藏中心

KBM5 T315I Cells

Cat No.: NTCC510625

Disease:     Chronic myelogenous leukemia, BCR-ABL1positive

Species of origin: Homo sapiens

Sex of cell:  Female

Category:    Cancer cell line

KBM-5 is a leukemiccell line derived from a patient in the blastic phase of chronic myelogenousleukemia. The cells exhibited multiple copies of the Philadelphia chromosome,and a high level of p210Bcr-Abl kinase activity was detected with rabbitanti-Abl and anti-Bcr (exon 3) peptide antisera. Use of specific primers andpolymerase chain reaction followed by Southern blotting revealed that KBM-5cells carried a bcr3-ABLII splice junction. While a normal BCR message wasdetected, no normal ABL message was found. The cells were phenotypicallymyeloid with monocytic differentiation. The high cloning efficiency insemisolid media was independent of the presence of exogenous colony-stimulatingfactors. In vitro exposure to induces of differentiation, such as retinoicacid, dimethyl sulfoxide, or hemin, failed to influence the growth rate of thecells and their level of differentiation. KBM-5 cells are highly resistant tothe antiproliferative action of recombinant alpha- and gamma-interferons.Although sensitive to recombinant tumor necrosis factor alpha, they werecompletely resistant to natural killer cell action. KBM-5 cells constitutivelyexpressed mRNA for tumor necrosis factor alpha but not for gamma-interferon,other interleukins, or hematopoietic growth factors. The KBM-5 cells that weretransplanted into SCID mice manifested metastatic potential and tissueinvasiveness similar to the way leukemic cells in humans do. This new KBM-5cell line represents a helpful model for examining in vitro and in vivomodulation of the growth and properties of leukemic cells by using biologicaland chemotherapeutic agents.

Medium: IMDM medium with 10% FBS(Gibco)

Cryopreservation medium:

Complete growth medium, 95%; DMSO, 5%

BioVector NTCC Inc.