HEK293puro-ISRE-Luc BioVector NTCC质粒载体菌种细胞基因保藏中心

Organism

Homo sapiens, human

Tissue

embryonic kidney

Morphology

epithelial

Culture Properties

adherent

Biosafety Level

2   [Cells contain adenovirus]

Age

fetus

Karyotype

HEK293puro-ISRE-luc is a reporter cell line in which firefly luciferase expression is driven by interferon stimulated response elements

HEK293 is a hypotriploid human cell line. The modal chromosome number was 64, occurring in 30% of cells. The rate of cells with higher ploidies was 4.2 %. The der(1)t(1;15) (q42;q13), der(19)t(3;19) (q12;q13), der(12)t(8;12) (q22;p13), and four other marker chromosomes were common to most cells. Five other markers occurred in some cells only. The marker der(1) and M8 (or Xq+) were often paired. There were four copies of N17 and N22. Noticeably in addition to three copies of X chromosomes, there were paired Xq+, and a single Xp+ in most cells.

Tumorigenic

YES

Effects

Yes, forms tumors in nude mice

Complete Growth Medium

The base medium for this cell line is Eagle's Minimum Essential Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%, 1μg/ml puromycin.