CRISPRi Gen1C 5 Induced pluripotent stem cell NTCC®细胞株/完全培养基-BioVector NTCC典型培养物保藏中心

[Designation名称] NTCC® CRISPRi Gen1C 5 Cell Line细胞株
BioVector NTCC Inc.

Cross-references: Wikidata; Q93458763
References identifiers: PubMed=26971820;
Synonyms: WTC CRISPRi Gen1 clone 5; CRISPRi (Gen1C 5); 1C-5
Comments: Population: Japanese.
Comments: Characteristics: Cell line with Dox-inducible CRISPR interference (CRISPRi).
Comments: Characteristics: Using TALEN a construct containing a rtTA+TRE3G promoter and dCas9-KRAB-P2A-mCherry is introduced in the AAVS1 locus.
Comments: Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (with p.Asp10Ala and p.His840Ala and KRAB domain = dCas9-KRAB).
Comments: Transfected with: UniProtKB; X5DSL3; Anaplasma marginale mCherry fluorescent protein.
Comments: Derived from site: In situ; Leg, skin; UBERON=UBERON_0001511.
Comments: Cell type: Fibroblast of skin; CL=CL_0002620.
Species of origin: NCBI_TaxID=9606; ! Homo sapiens (Human)
Hierarchy: CVCL_Y803 ! GM25256
Sex of cell: Male
[Organism物种来源]
[Disease疾病种类]
[Accession编号] NTCC® Accession: CVCL_VM38
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[Reference参考文献]
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[Date保藏时间]
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培养方法：
Medium培养基：NTCC® Specific Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Website网址：www.biovector.net