CRISPRi Gen1B 3 Induced pluripotent stem cell NTCC®细胞株/完全培养基-BioVector NTCC典型培养物保藏中心

[Designation名称] NTCC® CRISPRi Gen1B 3 Cell Line细胞株
BioVector NTCC Inc.

Cross-references: Wikidata; Q93458761
References identifiers: PubMed=26971820;
Synonyms: WTB CRISPRi Gen1 clone 3; 1B-3
Comments: Characteristics: Cell line with Dox-inducible CRISPR interference (CRISPRi).
Comments: Characteristics: Using TALEN a construct containing a rtTA+TRE3G promoter and dCas9-KRAB-P2A-mCherry is introduced in the AAVS1 locus.
Comments: Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (with p.Asp10Ala and p.His840Ala = dCas9).
Comments: Transfected with: UniProtKB; X5DSL3; Anaplasma marginale mCherry fluorescent protein.
Species of origin: NCBI_TaxID=9606; ! Homo sapiens (Human)
Hierarchy: CVCL_VM30 ! WTB6
Sex of cell: Female
Age of donor at sampling: Age unspecified
Category: Induced pluripotent stem cell
Date (entry history): Created: 07-09-18; Last updated: 30-01-24; Version: 5
[Organism物种来源]
[Disease疾病种类]
[Accession编号] NTCC® Accession: CVCL_VM36
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[Reference参考文献]
[Category分类]
[Date保藏时间]
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培养方法：
Medium培养基：NTCC® Specific Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Website网址：www.biovector.net