出品公司: | Invitrogen |
---|---|
载体名称: | pMT-Bip-V5-HisA |
质粒类型: | 昆虫细胞表达载体 |
高拷贝/低拷贝: | -- |
启动子: | P-MT (Metallothionein) |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 3642bp |
5' 测序引物及序列: | Metallothionein: 5'd[CATCTCAGTGCAACTAAA]3' |
3' 测序引物及序列: | -- |
载体标签: | BiP (N), V5 (C), His (C) |
载体抗性: | Ampicillin |
筛选标记: | -- |
备注: | 使用硫酸铜或氯化镉作为诱导剂。 |
产品目录号: | V413020 |
稳定性: | -- |
组成型: | 诱导型 |
病毒/非病毒: | 非病毒 |
pMT-Bip-V5-HisA质粒载体图谱序列抗性价格报价Biovector NTCC Inc.现货
- 价 格:¥1920
- 货 号:BiovectorpMT-Bip-V5-HisA
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
电话:400-800-2947 工作QQ:1843439339 (微信同号)
邮件:Biovector@163.com
手机:18901268599
地址:北京
- 已注册
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pMT/BIP/V5-HisA 3642 bp ds-DNA circular SYN 09-四月-2014 DEFINITION Drosophila vector for inducible high-level expression of secreted and tagged proteins. For other reading frames, use pMT/BiP/V5-His B or pMT/BiP/V5-His C. ACCESSION . VERSION . KEYWORDS pMT BiP V5-His A SOURCE synthetic DNA construct ORGANISM synthetic DNA construct COMMENT This file is created by Vector NTI http://www.biovector.net/ COMMENT VNTAUTHORNAME|biovector.net| COMMENT To create stable cell lines, co-transfect with the drug selection vector pCoHygro or pCoBlast. FEATURES Location/Qualifiers source 1..3642 /organism="synthetic DNA construct" /lab_host="Insect Cells" /mol_type="other DNA" promoter 411..833 /label="MT promoter" /label="Drosophila metallothionein promoter" CDS 851..904 /codon_start=1 /product="Drosophila BiP signal sequence" /label="BiP signal sequence" /translation="MKLCILLAVVAFVGLSLG" misc_feature 905..1002 /label="MCS" /label="multiple cloning site" CDS 1003..1044 /codon_start=1 /product="epitope tag from simian virus 5" /label="V5 tag" /translation="GKPIPNPLLGLDST" CDS 1054..1071 /codon_start=1 /product="6xHis affinity tag" /label="6xHis" /translation="HHHHHH" polyA_signal 1144..1278 /label="SV40 poly(A) signal" /label="SV40 polyadenylation signal" rep_origin complement(1824..2412) /direction=LEFT /label="ori" /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2583..3443) /codon_start=1 /gene="bla" /product="beta-lactamase" /label="AmpR" /label="confers resistance to ampicillin, carbenicillin, and related antibiotics" promoter complement(3444..3548) /gene="bla" /label="AmpR promoter" ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccag tgaattttaa cgttgcagga 421 caggatgtgg tgcccgatgt gactagctct ttgctgcagg ccgtcctatc ctctggttcc 481 gataagagac ccagaactcc ggccccccac cgcccaccgc cacccccata catatgtggt 541 acgcaagtaa gagtgcctgc gcatgcccca tgtgccccac caagagtttt gcatcccata 601 caagtcccca aagtggagaa ccgaaccaat tcttcgcggg cagaacaaaa gcttctgcac 661 acgtctccac tcgaatttgg agccggccgg cgtgtgcaaa agaggtgaat cgaacgaaag 721 acccgtgtgt aaagccgcgt ttccaaaatg tataaaaccg agagcatctg gccaatgtgc 781 atcagttgtg gtcagcagca aaatcaagtg aatcatctca gtgcaactaa aggggggatc 841 cgatctcaat atgaagttat gcatattact ggccgtcgtg gcctttgttg gcctctcgct 901 cgggagatct ccatggcccg gggtacctac tagtccagtg tggtggaatt ctgcagatat 961 ccagcacagt ggcggccgct cgagtctaga gggcccttcg aaggtaagcc tatccctaac 1021 cctctcctcg gtctcgattc tacgcgtacc ggtcatcatc accatcacca ttgagtttaa 1081 acccgctgat cagcctcgac tgtgccttct aaggcctgag ctcgctgatc agcctcgatc 1141 gaggatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag 1201 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata 1261 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg 1321 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat 1381 gatcagtcga cctgcaggca tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg 1441 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa 1501 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct 1561 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga 1621 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 1681 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 1741 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 1801 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 1861 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 1921 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 1981 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 2041 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 2101 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 2161 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 2221 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 2281 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 2341 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 2401 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 2461 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 2521 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 2581 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 2641 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 2701 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 2761 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 2821 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 2881 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 2941 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 3001 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 3061 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 3121 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 3181 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 3241 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 3301 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 3361 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 3421 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 3481 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 3541 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg 3601 acattaacct ataaaaatag gcgtatcacg aggccctttc gt
您正在向 biovector.net 发送关于产品 pMT-Bip-V5-HisA质粒载体图谱序列抗性价格报价Biovector NTCC Inc.现货 的询问
- 公告/新闻