pCA-Gag-Pro-Pol慢病毒包装质粒2代3代慢病毒包装适用-BioVector NTCC质粒载体菌种细胞基因保藏中心

pCA-Gag-Pro-Pol慢病毒包装质粒

2nd generation lentiviral packaging plasmid. Can be used with 2nd or 3rd generation lentiviral vectors and envelope expressing plasmid，Known to work with many of the Aebischer and Trono lab lentiviral vectors,and pLKO system.

Map：

Sequence：

gtcgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatat

ggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattg

acgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggact

atttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgt

caatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcag

tacatctacgtattagtcatcgctattaccatgggtcgaggtgagccccacgttctgcttcactctcccc

atctcccccccctccccacccccaattttgtatttatttattttttaattattttgtgcagcgatggggg

cggggggggggggggcgcgcgccaggcggggcggggcggggcgaggggcggggcggggcgaggcggagag

gtgcggcggcagccaatcagagcggcgcgctccgaaagtttccttttatggcgaggcggcggcggcggcg

gccctataaaaagcgaagcgcgcggcgggcgggagtcgctgcgttgccttcgccccgtgccccgctccgc

gccgcctcgcgccgcccgccccggctctgactgaccgcgttactcccacaggtgagcgggcgggacggcc

cttctcctccgggctgtaattagcgcttggtttaatgacggctcgtttcttttctgtggctgcgtgaaag

ccttaaagggctccgggagggccctttgtgcgggggggagcggctcggggggtgcgtgcgtgtgtgtgtg

cgtggggagcgccgcgtgcggcccgcgctgcccggcggctgtgagcgctgcgggcgcggcgcggggcttt

gtgcgctccgcgtgtgcgcgaggggagcgcggccgggggcggtgccccgcggtgcgggggggctgcgagg

ggaacaaaggctgcgtgcggggtgtgtgcgtgggggggtgagcagggggtgtgggcgcggcggtcgggct

gtaacccccccctgcacccccctccccgagttgctgagcacggcccggcttcgggtgcggggctccgtgc

ggggcgtggcgcggggctcgccgtgccgggcggggggtggcggcaggtgggggtgccgggcggggcgggg

ccgcctcgggccggggagggctcgggggaggggcgcggcggccccggagcgccggcggctgtcgaggcgc

ggcgagccgcagccattgccttttatggtaatcgtgcgagagggcgcagggacttcctttgtcccaaatc

tggcggagccgaaatctgggaggcgccgccgcaccccctctagcgggcgcgggcgaagcggtgcggcgcc

ggcaggaaggaaatgggcggggagggccttcgtgcgtcgccgcgccgccgtccccttctccatctccagc

ctcggggctgccgcagggggacggctgccttcgggggggacggggcagggcggggttcggcttctggcgt

gtgaccggcggctctagagcctctgctaaccatgttcatgccttcttctttttcctacagctcctgggca

acgtgctggttattgtgctgtctcatcattttggcaaagaattcgggccggccgcgttgacgcgcacggc

aagaggcgaggggcggcgactggtgagagatgggtgcgagagcgtcagtattaagcgggggagaattaga

tcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatataaattaaaacatatagtatgggca

agcagggagctagaacgattcgcagttaatcctggcctgttagaaacatcagaaggctgtagacaaatac

tgggacagctacaaccatcccttcagacaggatcagaagaacttagatcattatataatacagtagcaac

cctctattgtgtgcatcaaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagag

caaaacaaaagtaagaaaaaagcacagcaagcagcagctgacacaggacacagcaatcaggtcagccaaa

attaccctatagtgcagaacatccaggggcaaatggtacatcaggccatatcacctagaactttaaatgc

atgggtaaaagtagtagaagagaaggctttcagcccagaagtgatacccatgttttcagcattatcagaa

ggagccaccccacaagatttaaacaccatgctaaacacagtggggggacatcaagcagccatgcaaatgt

taaaagagaccatcaatgaggaagctgcagaatgggatagagtgcatccagtgcatgcagggcctattgc

accaggccagatgagagaaccaaggggaagtgacatagcaggaactactagtacccttcaggaacaaata

ggatggatgacacataatccacctatcccagtaggagaaatctataaaagatggataatcctgggattaa

ataaaatagtaagaatgtatagccctaccagcattctggacataagacaaggaccaaaggaaccctttag

agactatgtagaccgattctataaaactctaagagccgagcaagcttcacaagaggtaaaaaattggatg

acagaaaccttgttggtccaaaatgcgaacccagattgtaagactattttaaaagcattgggaccaggag

cgacactagaagaaatgatgacagcatgtcagggagtggggggacccggccataaagcaagagttttggc

tgaagcaatgagccaagtaacaaatccagctaccataatgatacagaaaggcaattttaggaaccaaaga

aagactgttaagtgtttcaattgtggcaaagaagggcacatagccaaaaattgcagggcccctaggaaaa

agggctgttggaaatgtggaaaggaaggacaccaaatgaaagattgtactgagagacaggctaatttttt

agggaagatctggccttcccacaagggaaggccagggaattttcttcagagcagaccagagccaacagcc

ccaccagaagagagcttcaggtttggggaagagacaacaactccctctcagaagcaggagccgatagaca

aggaactgtatcctttagcttccctcagatcactctttggcagcgacccctcgtcacaataaagataggg

gggcaattaaaggaagctctattagatacaggagcagatgatacagtattagaagaaatgaatttgccag

gaagatggaaaccaaaaatgatagggggaattggaggttttatcaaagtaggacagtatgatcagatact

catagaaatctgcggacataaagctataggtacagtattagtaggacctacacctgtcaacataattgga

agaaatctgttgactcagattggctgcactttaaattttcccattagtcctattgagactgtaccagtaa

aattaaagccaggaatggatggcccaaaagttaaacaatggccattgacagaagaaaaaataaaagcatt

agtagaaatttgtacagaaatggaaaaggaaggaaaaatttcaaaaattgggcctgaaaatccatacaat

actccagtatttgccataaagaaaaaagacagtactaaatggagaaaattagtagatttcagagaactta

ataagagaactcaagatttctgggaagttcaattaggaataccacatcctgcagggttaaaacagaaaaa

atcagtaacagtactggatgtgggcgatgcatatttttcagttcccttagataaagacttcaggaagtat

actgcatttaccatacctagtataaacaatgagacaccagggattagatatcagtacaatgtgcttccac

agggatggaaaggatcaccagcaatattccagtgtagcatgacaaaaatcttagagccttttagaaaaca

aaatccagacatagtcatctatcaatacatggatgatttgtatgtaggatctgacttagaaatagggcag

catagaacaaaaatagaggaactgagacaacatctgttgaggtggggatttaccacaccagacaaaaaac

atcagaaagaacctccattcctttggatgggttatgaactccatcctgataaatggacagtacagcctat

agtgctgccagaaaaggacagctggactgtcaatgacatacagaaattagtgggaaaattgaattgggca

agtcagatttatgcagggattaaagtaaggcaattatgtaaacttcttaggggaaccaaagcactaacag

aagtagtaccactaacagaagaagcagagctagaactggcagaaaacagggagattctaaaagaaccggt

acatggagtgtattatgacccatcaaaagacttaatagcagaaatacagaagcaggggcaaggccaatgg

acatatcaaatttatcaagagccatttaaaaatctgaaaacaggaaaatatgcaagaatgaagggtgccc

acactaatgatgtgaaacaattaacagaggcagtacaaaaaatagccacagaaagcatagtaatatgggg

aaagactcctaaatttaaattacccatacaaaaggaaacatgggaagcatggtggacagagtattggcaa

gccacctggattcctgagtgggagtttgtcaatacccctcccttagtgaagttatggtaccagttagaga

aagaacccataataggagcagaaactttctatgtagatggggcagccaatagggaaactaaattaggaaa

agcaggatatgtaactgacagaggaagacaaaaagttgtccccctaacggacacaacaaatcagaagact

gagttacaagcaattcatctagctttgcaggattcgggattagaagtaaacatagtgacagactcacaat

atgcattgggaatcattcaagcacaaccagataagagtgaatcagagttagtcagtcaaataatagagca

gttaataaaaaaggaaaaagtctacctggcatgggtaccagcacacaaaggaattggaggaaatgaacaa

gtagatgggttggtcagtgctggaatcaggaaagtactatttttagatggaatagataaggcccaagaag

aacatgagaaatatcacagtaattggagagcaatggctagtgattttaacctaccacctgtagtagcaaa

agaaatagtagccagctgtgataaatgtcagctaaaaggggaagccatgcatggacaagtagactgtagc

ccaggaatatggcagctagattgtacacatttagaaggaaaagttatcttggtagcagttcatgtagcca

gtggatatatagaagcagaagtaattccagcagagacagggcaagaaacagcatacttcctcttaaaatt

agcaggaagatggccagtaaaaacagtacatacagacaatggcagcaatttcaccagtactacagttaag

gccgcctgttggtgggcggggatcaagcaggaatttggcattccctacaatccccaaagtcaaggagtaa

tagaatctatgaataaagaattaaagaaaattataggacaggtaagagatcaggctgaacatcttaagac

agcagtacaaatggcagtattcatccacaattttaaaagaaaaggggggattggggggtacagtgcaggg

gaaagaatagtagacataatagcaacagacatacaaactaaagaattacaaaaacaaattacaaaaattc

aaaattttcgggtttattacagggacagcagagatccagtttggaaaggaccagcaaagctcctctggaa

aggtgaaggggcagtagtaatacaagataatagtgacataaaagtagtgccaagaagaaaagcaaagatc

atcagggattatggaaaacagatggcaggtgatgattgtgtggcaagtagacaggatgaggattaacaca

tggaattctgcaacaactgctgtttatccatttcagaattgggtgtcgacatagcagaataggcgttact

cgacagaggagagcaagaaatggagccagtagatcctagactagagccctggaagcatccaggaagtcag

cctaaaactgcttgtaccaattgctattgtaaaaagtgttgctttcattgccaagtttgtttcatgacaa

aagccttaggcatctcctatggcaggaagaagcggagacagcgacgaagagctcatcagaacagtcagac

tcatcaagcttctctatcaaagcagtaagtagtacatgtaatgcaacctataatagtagcaatagtagca

ttagtagtagcaataataatagcaatagttgtgtggtccatagtaatcatagaatataggaaaatggccg

ctgatcttcagacctggaggaggagatatgagggacaattggagaagtgaattatataaatataaagtag

taaaaattgaaccattaggagtagcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagc

agtgggaataggagctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcctcaatg

acgctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggcta

ttgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaagcaagaatcctagc

tgtggaaagatacctaaaggatcaacagctcctagggatttggggttgctctggaaaactcatttgcacc

actgctgtgccttggaatgctagttggagtaataaatctctggaacagatctggaatcacacgacctgga

tggagtgggacagagaaattaacaattacacaagcttaatacactccttaattgaagaatcgcaaaacca

gcaagaaaagaatgaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacata

acaaattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaagaatagttt

ttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcagacccacctccc

aatcccgaggggacccgacaggcccgaaggaatagaagaagaaggtggagagagagacagagacagatcc

attcgattagtgaacggatccttggcacttatctgggacgatctgcggagcctgtgcctcttcagctacc

accgcttgagagacttactcttgattgtaacgaggattgtggaacttctgggacgcagggggtgggaagc

cctcaaatattggtggaatctcctacaatattggagtcaggagctaaagaatagtgctgttagcttgctc

aatgccacagccatagcagtagctgaggggacagatagggttatagaagtagtacaaggagcttgtagag

ctattcgccacatacctagaagaataagacagggcttggaaaggattttgctataagctcgaaacaaccg

gtacctctagaactatagctagcagatctttttccctctgccaaaaattatggggacatcatgaagcccc

ttgagcatctgacttctggctaataaaggaaatttattttcattgcaatagtgtgttggaattttttgtg

tctctcactcggaaggacatatgggagggcaaatcatttaaaacatcagaatgagtatttggtttagagt

ttggcaacatatgccatatgctggctgccatgaacaaaggtggctataaagaggtcatcagtatatgaaa

cagccccctgctgtccattccttattccatagaaaagccttgacttgaggttagattttttttatatttt

gttttgtgttatttttttctttaacatccctaaaattttccttacatgttttactagccagatttttcct

cctctcctgactactcccagtcatagctgtccctcttctcttatgaagatccctcgacctgcagcccaag

cttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacata

cgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgc

gctcactgcccgctttccagtcgggaaacctgtcgtgccagcggatccgcatctcaattagtcagcaacc

atagtcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgccccatg

gctgactaattttttttatttatgcagaggccgaggccgcctcggcctctgagctattccagaagtagtg

aggaggcttttttggaggcctaggcttttgcaaaaagctaacttgtttattgcagcttataatggttaca

aataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtc

caaactcatcaatgtatcttatcatgtctggatccgctgcattaatgaatcggccaacgcgcggggagag

gcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg

gcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaag

aacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccata

ggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggact

ataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttacc

ggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcaatgctcacgctgtaggtatctca

gttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgc

cttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccact

ggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacg

gctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttgg

tagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacg

cgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaa

actcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaa

atgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagt

gaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataa

ctacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggc

tccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatcc

gcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgca

acgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccgg

ttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcct

ccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctc

ttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaata

gtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaact

ttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagat

ccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgg

gtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactc

atactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttg

aatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctg

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