出品公司: | Invitrogen |
---|---|
载体名称: | pFastBacHTB, pFastBac HTB, pFastBacHT B, pFastBac HT B |
质粒类型: | 昆虫细胞表达载体 |
启动子: | Polyhedrin |
表达水平: | 高 |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 4857 bp |
5' 测序引物: | pFastbac-F: TATTCCGGATTATTCATACC |
3' 测序引物: | pFastBac-R: ACAAATGTGGTATGGCTGA |
载体标签: | N-His, N-TEV |
载体抗性: | Ampicillin 氨苄 |
筛选标记: | Gentamicin 庆大霉素 |
备注: | |
产品目录号: | 10584-027 |
稳定性: | 稳表达 Stable |
组成型: | 组成型 Constitutive |
病毒/非病毒: | 杆状病毒 |
pFastBacHT B质粒载体图谱序列抗性价格报价Biovector NTCC Inc.现货
- 价 格:¥1920
- 货 号:BiovectorpFastBacHT B
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
电话:400-800-2947 工作QQ:1843439339 (微信同号)
邮件:Biovector@163.com
手机:18901268599
地址:北京
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pFastBacHTB载体基本信息
pFastBacHTB载体质粒图谱和多克隆位点信息
pFastBacHTB载体简介
The pFastBac HT vector is supplied with the multiple cloning site in three reading frames (A, B, and C) to facilitate cloning your gene of interest in frame with the N-terminal 6xHis tag.
• Strong polyhedrin (PH) promoter for high-level protein expression.
• N-terminal 6xHis tag for purification of recombinant fusion proteins using metal-chelating resin and a TEV protease cleavage site for removal of the 6xHis tagfollowing protein purification.
• Vector supplied in 3 reading frames for simplified cloning.
pFastBacHTB载体序列
LOCUS pFastBacTHT\B 4857 bp DNA circular SYN 2-SEP-2003 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.biovector.net/ COMMENT VNTAUTHORNAME|biovector,net| FEATURES Location/Qualifiers source 1..4857 /invitrogen="607" /vntifkey="98" CDS 589..1449 /invitrogen="1020000" /vntifkey="4" /label=Amp(R) promoter 490..588 /invitrogen="2330000" /vntifkey="30" /label=bla\promoter rep_origin 2..457 /invitrogen="2520000" /vntifkey="33" /label=f1\origin CDS complement(2802..3335) /invitrogen="1150000" /vntifkey="4" /label=Gm(R) misc_feature 4119..4223 /invitrogen="1530000" /vntifkey="21" /label=MCS promoter complement(3524..3551) /invitrogen="2250000" /vntifkey="29" /label=Pc\promoter promoter 3904..4032 /invitrogen="2260000" /vntifkey="29" /label=PH\promoter primer 3920..3937 /invitrogen="2060000" /vntifkey="27" /label=Polyhedrin\forward\primer rep_origin 1594..2267 /invitrogen="2540000" /vntifkey="33" /label=pUC\origin polyA_signal 4241..4481 /invitrogen="1850000" /vntifkey="25" /label=SV40\pA CDS 4101..4121 /invitrogen="1300000" /vntifkey="4" /label=TEV\recognition\site misc_recomb 4500..4694 /invitrogen="2750000" /vntifkey="86" /label=Tn7L misc_recomb 2512..2735 /invitrogen="2760000" /vntifkey="86" /label=Tn7R CDS 4062..4079 /invitrogen="1015000" /vntifkey="4" /label=6xHis CDS 4050..4052 /invitrogen="123000000" /vntifkey="4" /label=initiation\ATG BASE COUNT 1219 a 1198 c 1152 g 1288 t ORIGIN 1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc 61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc 121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt 181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg 241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt 301 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta 361 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt 421 aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat 481 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg 541 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa 601 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac 661 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac 721 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt 781 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc 841 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca 901 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc 961 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag 1021 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa 1081 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg 1141 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa 1201 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg 1261 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt 1321 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt 1381 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag 1441 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat 1501 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct 1561 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct 1621 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca 1681 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc 1741 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc 1801 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct 1861 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag 1921 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc 1981 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg 2041 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag 2101 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt 2161 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac 2221 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg 2281 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc 2341 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg 2401 cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct 2461 ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga 2521 caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag 2581 acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt 2641 tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga 2701 ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac 2761 aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg 2821 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg 2881 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca 2941 tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact 3001 gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc 3061 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta 3121 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct 3181 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg 3241 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg 3301 ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca 3361 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa 3421 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa 3481 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca 3541 ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac 3601 cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc 3661 ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg 3721 cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt 3781 ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt 3841 gttcgcccag gactctagct atagttctag tggttggcta cgtatactcc ggaatattaa 3901 tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt 3961 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca 4021 ccatcgggcg cggatctcgg tccgaaacca tgtcgtacta ccatcaccat caccatcacg 4081 attacgatat cccaacgacc gaaaacctgt attttcaggg cgccatggga tccggaattc 4141 aaaggcctac gtcgacgagc tcactagtcg cggccgcttt cgaatctaga gcctgcagtc 4201 tcgaggcatg cggtaccaag cttgtcgaga agtactagag gatcataatc agccatacca 4261 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac 4321 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat 4381 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg 4441 gtttgtccaa actcatcaat gtatcttatc atgtctggat ctgatcactg atatcgccta 4501 ggagatccga accagataag tgaaatctag ttccaaacta ttttgtcatt tttaattttc 4561 gtattagctt acgacgctac acccagttcc catctatttt gtcactcttc cctaaataat 4621 ccttaaaaac tccatttcca cccctcccag ttcccaacta ttttgtccgc ccacagcggg 4681 gcatttttct tcctgttatg tttttaatca aacatcctgc caactccatg tgacaaaccg 4741 tcatcttcgg ctactttttc tctgtcacag aatgaaaatt tttctgtcat ctcttcgtta 4801 ttaatgtttg taattgactg aatatcaacg cttatttgca gcctgaatgg cgaatgg //
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