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pRAF800
功能:
乳酸菌食品级表达载体
图谱Map:
Genetic organization of pRAF800. (A) Sequence of the replication origin of pRAF800. The replicon of the L. lactis plasmid pSRQ800 was used to construct pRAF800. The nucleotide boxed in black differs from pSRQ800 (T→G substitution). Direct repeats (DR) are underlined (continuous and discontinuous). Inverted repeats (IR) are indicated in boldface. The putative −35 and −10 boxes of the repB promoter are shaded. The repB start codon is italicized. (B) Map of pRAF800. Genes are identified by shaded arrows oriented to indicate the direction of transcription. aga, gene encoding α-galactosidase; repB, gene encoding the replication initiator protein. The positions of primers used for RT-PCR are indicated on the plasmid map. bp, base pairs.
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