pSH26， pSH27 plasmid-BioVector NTCC质粒载体菌种细胞基因保藏中心

BioVector® NTCC® pSH26， pSH27

pFC53 mouse Airn promoter region 2 Dr. F. Chédin (ref. (Ginno et al., 2012) )pM49.2 - Dr. J. Griesenbeck (ref. (Griesenbeck et al., 2004)pcDNA3.1 Hygro (+) - Laboratory collectionpLVXtight Puro ∆N1-27RH-FLAG ∆N1-27 RNase H1-FLAG Dr. C. Stork (ref. (Sollier et al., 2014) )pcDNA3.1 ∆N1-27 RHFLAG NotI-∆N1-27 RNase H1-FLAG-EcoRINotI/EcoRI fragment from pLVXtight Puro ∆N1-27RH-FLAG blunted and cloned into pcDNA3.1Hygro (+) cut with EcoRVpEco3∆ TRE ECFPbeta-Actin - Dr. Julie SollierpBlueSkript 3xLEXA AflIII-3xLEXA-AflIII Insertion of AflIII cut SH34/SH35 PCR ampliconfrom pM49.2 into pBlueSkript II SK(-) cut with AflIIIpBlueSkript 3xLEXAECFP KpnI-ECFP-XhoI KpnI/XhoI fragment from pEco3∆ TRE ECFP-betaActin into pBlueSkript 3xLEXA cut with KpnI/XhoIpSH24 KpnI-pTRE_tight-BsrGIInsertion of KpnI/BsrGI cut SH11/SH12 PCRamplicon from pEco3∆ TRE ECFP-beta-Actin intopCEP4 cut with KpnI/BsrGIpSH24 1xLEXA BglII-3xLEXA-BglII Insertion of BglII cut SH13/SH14 PCR ampliconfrom pM49.2 into pSH24 cut with BglIIpSH24 2xLEXA BglII-3xLEXA-BglII Insertion of blunted SH13/SH14 PCR ampliconfrom pM49.2 into pSH24 1xLEXA cut with NruIpSH25 2xLEXA -SalI fragment from pSH24 2xLEXA reinserted inpSH24 2xLEXA in opposite directionpSH26 KpnI-ECFP-XhoI KpnI/XhoI fragment from pEco3∆ TRE ECFP-betaActin into pSH24 2xLEXA cut with KpnI/XhoIpSH27 KpnI-ECFP-XhoI KpnI/XhoI fragment from pEco3∆ TRE ECFP-betaActin into pSH25 2xLEXA cut with KpnI/XhoIpBlueSkript 3xLEXAmAIRN KpnI-mAIRN-BamHI KpnI/BamHI fragment from pFC53 into pBlueSkript3xLEXA cut with KpnI/BamHIpBlueSkript 3xLEXAmAIRN_rep StyI-CGAACTCCAGCAGGACCATGT- StyISH24/SH25 annealed and cloned into pBlueSkript3xLEXA mAIRN cut with StyIpSH36 KpnI-mAIRN-BamHIKpnI/BamHI fragment from pBlueSkript 3xLEXAmAIRN_rep into pSH24 2xLEXA cut withKpnI/BamHIpSH37 KpnI-mAIRN-BamHIKpnI/BamHI fragment from pBlueSkript 3xLEXAmAIRN_rep into pSH25 2xLEXA cut withKpnI/BamHI

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