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BioVector® NTCC® pYL84
An NF-κB inducible SEAP expression vector driven by a promoter consisting of ((NF-κB'-NF-κB)2-NF-κB')2 binding sites and an IFNB promoter (P[NF-κB'-NF-κB]2-IFNβmin-SEAP-pA). ((NF-κB'-NF-κB)2-NF-κB')2 was annealed through OYL164 (5’-CTCGAGGCTAGCTGGGAATTTCCGGGGACTTTCCGGGAATTTCCGGGGACTTTCCGGGAATTTCCGGGAATTTCCGGGGACTTTCCGGGAATTTCCGGGGACTTTCCGGGAATTTCC-3’, NheI underlined) and OYL165 (5’-TATTCAAGCCTGCAGGAGATCTAGTGGAAATTCCCGGAAAGTCCCCGGAAATTCCCGGAAAGTCCCCGGAAATTCCCGGAAATTCCCGGAAAGTCCCCGGAAATTCCCGGAAA-3’, SbfI underlined), digested with NheI/SbfI and inserted into corresponding sites of pYL3.
Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net
An NF-κB inducible SEAP expression vector driven by a promoter consisting of ((NF-κB'-NF-κB)2-NF-κB')2 binding sites and an IFNB promoter (P[NF-κB'-NF-κB]2-IFNβmin-SEAP-pA). ((NF-κB'-NF-κB)2-NF-κB')2 was annealed through OYL164 (5’-CTCGAGGCTAGCTGGGAATTTCCGGGGACTTTCCGGGAATTTCCGGGGACTTTCCGGGAATTTCCGGGAATTTCCGGGGACTTTCCGGGAATTTCCGGGGACTTTCCGGGAATTTCC-3’, NheI underlined) and OYL165 (5’-TATTCAAGCCTGCAGGAGATCTAGTGGAAATTCCCGGAAAGTCCCCGGAAATTCCCGGAAAGTCCCCGGAAATTCCCGGAAATTCCCGGAAAGTCCCCGGAAATTCCCGGAAA-3’, SbfI underlined), digested with NheI/SbfI and inserted into corresponding sites of pYL3.
Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net
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