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BioVector® NTCC® pYL72
PhCMV-driven expression of hGLuc-Gln125,232-lysostaphin C-terminally fused to human IgG-Fc (PhCMV-hGLuc-Lys-hFc-pA). hGLuc-Lys was PCR amplified from pYL48 using OYL138 (5’-CCCCCCAAGCTTCCACCATGGGAGTCAAAGTTCTGTTTGCCCTGATCTGCATCGCTGTGGCCGAGGCCGCTACTCACGAACACTC-3’, HindIII underlined) and OYL105 (5’-TGATCCGCCGCCACCCGACCCACCTCCGCCCGAGCCTCCGCCACCAGATTTGATAGTGCCCCA-3’, linker sequence underlined), and then annealed with human IgG-Fc, which was PCR amplified from pHY79 using OYL149 (5’-GGCGGAGGTGGGTCGGGTGGCGGCGGATCAATATCGGCCATGGTTAGA-3’) and OYL150 (5’-GGGCCCTCTAGAGAATTCTTATTTACCCGGAGACAGGG-3’, XbaI underlined), to create hGLuc-Lys-hFc. hGLuc-Lys-hFc was subsequently digested and cloned into pcDNA3.1(+) (HindIII/XbaI).
Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net
PhCMV-driven expression of hGLuc-Gln125,232-lysostaphin C-terminally fused to human IgG-Fc (PhCMV-hGLuc-Lys-hFc-pA). hGLuc-Lys was PCR amplified from pYL48 using OYL138 (5’-CCCCCCAAGCTTCCACCATGGGAGTCAAAGTTCTGTTTGCCCTGATCTGCATCGCTGTGGCCGAGGCCGCTACTCACGAACACTC-3’, HindIII underlined) and OYL105 (5’-TGATCCGCCGCCACCCGACCCACCTCCGCCCGAGCCTCCGCCACCAGATTTGATAGTGCCCCA-3’, linker sequence underlined), and then annealed with human IgG-Fc, which was PCR amplified from pHY79 using OYL149 (5’-GGCGGAGGTGGGTCGGGTGGCGGCGGATCAATATCGGCCATGGTTAGA-3’) and OYL150 (5’-GGGCCCTCTAGAGAATTCTTATTTACCCGGAGACAGGG-3’, XbaI underlined), to create hGLuc-Lys-hFc. hGLuc-Lys-hFc was subsequently digested and cloned into pcDNA3.1(+) (HindIII/XbaI).
Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net
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